大鼠脑微血管内皮细胞的体外培养与鉴定

doi:10.11843/j.issn.0366-6964.2014.08.008

Abstract

Abstract:

To establish a simple and feasible method for isolation of highly purified rats brain microvascular endothelial cells（RBMECs）from 7-10 days SD rats，the relatively pure cerebral microvessel fragments were obtained by careful dissection in dispase II and three times centrifugation with dextran.After digested with collagenase/dispase enzyme，cells were grown in a humidified atmosphere of 5% CO2 at 37 ℃,then were identificated by the factor Ⅷ-related antigen.The results showed that primary endothelium cells formed monolayers with the characteristic cobble-stone morphology and were strongly positive for Ⅷ-related antigen factor.It was indicated that relatively pure primary culture of RBMECs was successfully established using this method.

CLC Number:

S813.1

WANG Xin，FENG Bo，LIU Xiao-ye，ZHANG Qian，DONG Hong，MU Xiang. Isolation and Cultivation of Rat Brain Microvascular Endothelial Cells in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1260-1264.

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References

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Isolation and Cultivation of Rat Brain Microvascular Endothelial Cells in vitro

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